معلومات المقرر

Analytical Biochemistry is a course designed for Master 1 students in Applied Biochemistry. It introduces the fundamental principles and applications of analytical methods used to identify, characterize, and quantify biochemical compounds in different samples.

The course focuses on the main spectroscopic techniques used in biochemical analysis, including Ultraviolet–Visible (UV–Vis) spectrophotometry, Infrared (IR) spectroscopy, and Nuclear Magnetic Resonance (NMR) spectroscopy. It also covers atomic absorption spectrophotometry and emission spectrometry using a flame photometer.

Throughout the course, students explore the interaction between electromagnetic radiation and matter, the operating principles of spectroscopic instruments, and the analysis and interpretation of spectroscopic data. Particular attention is given to quantitative analysis using the Beer–Lambert law, the identification of functional groups through IR spectroscopy, and the interpretation of proton NMR spectra.

The module combines theoretical lectures, practical work, application exercises, group projects, and distance-learning activities. These learning activities aim to develop students’ theoretical knowledge, experimental skills, analytical thinking, scientific reasoning, and ability to apply spectroscopic techniques to biochemical problems.

Learning Objectives

At the end of the module, students should be able to:

ü  Explain the fundamental principles of major spectroscopic techniques and their interaction with electromagnetic radiation.

ü  Describe spectroscopic instrumentation and the operating principles.

ü  Apply spectroscopic methods for the qualitative and quantitative analysis of biochemical compounds, including the Beer–Lambert law.

ü  Analyze and interpret spectra obtained through UV–Visible, IR, and NMR spectroscopy to identify functional groups and characterize molecular structures.

ü  Develop practical and analytical skills by processing experimental data, interpreting spectroscopic results, and applying spectroscopic techniques to biochemical problems.